Autologous Fat Grafting: A comparison study of four current commercial protocols

Publication date: Available online 14 December 2016
Source:Journal of Plastic, Reconstructive & Aesthetic Surgery
Author(s): Vincent Hivernaud, Bruno Lefourn, Myriam Robard, Jérôme Guicheux, Pierre Weiss
BackgroundAutologous Fat Grafting is a widely used technique that gives natural results when treating soft tissue deficiencies. However, there is still no consensus on which is the best procedure to use, leading to unpredictable results due to fat graft resorption.ObjectivesThis study compared 4 commercial lipotransfer devices by analyzing the behavior of processed adipose tissue and the outcome of the adipose graft in an in vivo model.Methods4 different protocols that used manual, power or water assisted lipoaspiration and then decantation, centrifugation or filtration were used on each of 8 patients to process lipoaspirate. Harvested adipose tissue was assessed in vitro (tissue resorption, oil formation, cytokine secretion). Graft resorption rate was calculated and histological analyses were performed after subcutaneous injection in a murine model.ResultsAll protocols resulted in very low oil formation and histologically healthy grafts. The tissue volume was significantly greater after 2 days in culture when using manual lipoaspiration and soft centrifugations/washing steps (Microfill^®/Macrofill^®) compared to Water-Assisted Lipoaspiration/Decantation (BodyJet^®) and Power-Assisted Lipoaspiration/Filtration (PAL^®+PureGraft^®). These results were confirmed in mice one month after subcutaneous injection, with greater efficiency obtained with protocols that employed (A) manual aspiration, (B) soft centrifugations and (C) washing steps.ConclusionsWe have confirmed that the choice of technique used to process adipose tissue during lipotransfer surgery can highly influence fat grafting efficacy. In our study, the use of manual aspiration combined to soft centrifugations lead to the best results in the selected models.



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