Introduction of cationic virosome derived from vesicular stomatitis virus as a novel gene delivery system for sf9 cells.

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Introduction of cationic virosome derived from vesicular stomatitis virus as a novel gene delivery system for sf9 cells.

J Liposome Res. 2016 Mar 16;:1-7

Authors: Mohammadzadeh Y, Gholami S, Rasouli N, Sarrafzadeh S, Seyed Tabib NS, Samiee Aref MH, Abdoli A, Biglari P, Fotouhi F, Farahmand B, Tavassoti Kheiri M, Jamali A

Abstract
Insect-derived cell lines are used extensively to produce recombinant proteins because they are capable of performing a range of post-translational modifications. Due to their significance in biotechnological applications, various methods have been developed to transfect them. In this study, we introduce a virosome constructed from vesicular stomatitis virus (VSV) as a new delivery system for sf9 cells. We labeled these VSV virosomes by fluorescent probe Rhodamine B chloride (R18). By fluorescence microscope observation and conducting a fusion assay, we confirmed the uptake of VSV virosomes via endocytosis by sf9 cells and their fusion with the endosomal membrane. Moreover, we incubated cationic VSV virosomes with a GFP-expressing bacmid and transfected sf9 cells, after 24 h some cells expressed GFP indicating the ability of VSV virosomes to deliver heterologous DNA to these cells. This is the first report of a virosome-based delivery system introduced for an insect cell line.

PMID: 26981843 [PubMed - as supplied by publisher]

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