microRNA-21 and microRNA-375 from oral cytology as biomarkers for oral tongue cancer detection

Publication date: June 2016
Source:Oral Oncology, Volume 57
Author(s): Qianting He, Zujian Chen, Robert J. Cabay, Leitao Zhang, Xianghong Luan, Dan Chen, Tianwei Yu, Anxun Wang, Xiaofeng Zhou
ObjectiveWe previously performed a meta-analysis of microRNA profiling studies on head and neck/oral cancer (HNOC), and identified 11 consistently dysregulated microRNAs in HNOC. Here, we evaluate the diagnostic values of these microRNAs in oral tongue squamous cell carcinoma (OTSCC) using oral cytology samples.Materials and methodsThe levels of 11 microRNAs were assessed in 39 oral cytology samples (19 OTSCC and 20 normal subjects), and 10 paired OTSCC and adjacent normal tissues. The predictive power of these microRNAs was analyzed by receiver operating characteristic curve (ROC) and random forest (RF) model. A classification and regression trees (CART) model was generated using miR-21 and miR-375, and further validated using both independent oral cytology validation sample set (14 OTSCC and 11 normal subjects) and tissue validation sample set (12 paired OTSCC and adjacent normal tissues).ResultsDifferential expression of miR-21, miR-100, miR-125b and miR-375 was validated in oral cytology training sample set. Based on the RF model, the combination of miR-21 and miR-375 was selected which provide best prediction of OTSCC. A CART model was constructed using miR-21 and miR-375, and was tested in both oral cytology and tissue validation sample sets. A sensitivity of 100% and specificity of 64% was achieved in distinguishing OTSCC from normal in the oral cytology validation set, and a sensitivity of 83% and specificity of 83% was achieved in the tissue validation set.ConclusionThe utility of microRNA from oral cytology samples as biomarkers for OTSCC detection is successfully demonstrated in this study.



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