Σάββατο 29 Οκτωβρίου 2016

Therapeutic reversal of food allergen sensitivity by mature retinoic acid–differentiated dendritic cell induction of LAG3+CD49b−Foxp3− regulatory T cells

Publication date: Available online 26 October 2016
Source:Journal of Allergy and Clinical Immunology
Author(s): Wojciech Dawicki, Chunyan Li, Jennifer Town, Xiaobei Zhang, John R. Gordon
BackgroundAnaphylaxis is a life-threatening condition for which we have limited therapeutic options. Although specific immunotherapy for food allergies is becoming more effective, it is still laborious and carries substantial risk of adverse events. On the other hand, regulatory dendritic cell (DC) therapy is effective in mouse models of allergic disease and has been shown to work with TH2 cells from atopic asthmatic patients.ObjectiveWe assessed whether DC immunotherapy could reverse food allergen sensitivity in mouse models to provide proof of concept relating to their use in the clinic.MethodsWe generated and characterized mature retinoic acid–skewed dendritic cells (DC-RAs) and assessed their abilities to reverse ovalbumin or peanut allergies in mouse models, as well as their operative mechanisms.ResultsDC-RAs displayed a mature yet tolerogenic phenotype, expressing IL-10, TGF-β, IL-27, and aldehyde dehydrogenase 1A2 but not IL-12 or IL-35; IL-10 and TGF-β together drove their suppression of TH2 cell proliferation. Delivery of specific allergen-presenting DC-RAs to half-maximally sensitized mice with ovalbumin or peanut allergy reduced anaphylactic responses to oral allergen challenge by 84% to 90%, as well as diarrhea, mast cell activation, and TH2 cytokine responses and serum allergen-specific IgE/IgG1 levels. DC-RA expression of IL-27 was important to their induction of CD25+ lymphocyte activation gene 3 (LAG3)+, CD49b, forkhead box P3 (Foxp3) regulatory T cells in vitro, such that β subunit of IL-27 (Ebi)−/− (ie, IL-27–incompetent) DC-RAs were ineffective in inducing food allergen tolerance.ConclusionOur data indicate that regulatory DC immunotherapy can be effective for food allergies and suggest that induction of Foxp3 regulatory T cells might be a useful strategy for tolerance induction in this context.

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