Source:Journal of Oral and Maxillofacial Surgery
Author(s): Ling-fei Ren, Geng-sheng Shi, Yong-qing Tong, Song-yao Jiang, Feng Zhang
PurposeThe effects of a rhBMP-2/7 heterodimer and a RADA16 (Ac-RADARADARADARADA-CONH2) hydrogel scaffold on bone formation during distraction osteogenesis were evaluated.MethodsForty New Zealand white rabbits, which underwent mandibular lengthening, were randomly divided into five groups. One group served as a control group. The others received 2 μg rhBMP-2 homodimer, 2 μg rhBMP-2/7 heterodimer, 100 μL RADA16 or 100 μl RADA16+2 μg rhBMP-2/7 heterodimer in the mandibular distraction gap at the beginning of distraction. [18F-] fluoride positron emission tomography (PET) was used to assess osteogenesis both after distraction and at the end of consolidation. Dual-energy X-ray absorptiometer (DEXA) examination and bone histology were also evaluated.ResultsAt the end of distraction, the radioactivity concentration in the distracted area was significantly higher in the RADA16+ rhBMP-2/7 heterodimer group than the other groups (p<0.01); differences between the other four groups were also statistically significant in the following order: rhBMP-2/7 heterodimer group > rhBMP-2 homodimer group > RADA16 group (or control group) (p<0.05). However, the radioactivity concentration of the RADA16 group was slightly higher than that of the control group with a non-significant difference (p>0.05). By the end of consolidation, activity in the control group, RADA16 group, rhBMP-2 homodimer group and rhBMP-2/7 heterodimer group had significantly diminished (p<0.05); however, the activity in the RADA16+ rhBMP-2/7 heterodimer group remained at the same level (p>0.05). DEXA results and bone histology findings indicated that more callus regeneration was noted in the RADA16+ rhBMP-2/7 heterodimer group than any other group.ConclusionRhBMP-2/7 heterodimer and RADA16 hydrogel scaffold significantly promote mandibular distraction osteogenesis.
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