Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections.

Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections.

Cytotechnology. 2016 Apr 27;

Authors: Kidani S, Okuzaki Y, Kaneoka H, Asai S, Murakami S, Murase Y, Iijima S, Nishijima KI

Abstract
In mammals, interferon-inducible transmembrane proteins (IFITMs) prevent infections by various enveloped viruses. The expression of IFITMs in chicken was herein examined in the adult and embryonic organs using a quantitative reverse-transcription-polymerase chain reaction. The results obtained revealed that IFITM3 was expressed at a higher level than IFITM1, 2 and 5, in both embryonic and adult organs. However, the expression levels of IFITMs in embryonic organs were less than 5 % of those in adult lungs. Among the embryonic tissues examined, primordial germ cells (PGCs) at day 2.5 expressed relatively higher levels of IFITM3. IFITM3 expression levels were 1.5-fold higher in the chicken cell line DF-1 than in PGCs. The knockdown of IFITM3 in DF-1 cells by siRNA increased the infectivity of a vesicular stomatitis virus G protein-pseudotyped lentiviral vector, suggesting that lower levels of IFITM3 are still sufficient to restrict this viral vector.

PMID: 27117301 [PubMed - as supplied by publisher]

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