Photodynamic Therapy Associated with a Blue Dye Papain-Based Gel and Evaluation of Its Degradation of Type I Collagen Fibers.

Photodynamic Therapy Associated with a Blue Dye Papain-Based Gel and Evaluation of Its Degradation of Type I Collagen Fibers.

Photomed Laser Surg. 2017 Oct 12;:

Authors: Botta SB, Ana PA, Gonçalves MLL, Fernandes KPS, Agnelli Mesquita-Ferrari R, Prates RA, Brugnera Junior A, Bussadori SK

Abstract
OBJECTIVE: The aim of this in vitro study was to evaluate the degradation of type I collagen fibers after treatment with a papain-based gel associated with a blue dye (PapaMBlue™) for use in antimicrobial photodynamic therapy.
MATERIALS AND METHODS: For such, 60 bioabsorbable membrane sponge discs were used. Group 1 was the negative control group. In groups 2, 3, and 4, the papain-based gel PapaMBlue gel was applied all over the samples for 4 min and irradiated using red laser (660 ± 10 nm) with 15, 30, and 40 J/cm(2), respectively. In group 5, the papain-based gel was applied all over the samples for 4 min. In group 6, the photosensitizing dye was applied all over the samples for 4 min. The compositional analysis of the samples was performed using ATR-FTIR (attenuated total reflectance-Fourier transformed infrared spectroscopy). The data were statistically analyzed using ANOVA and Tukey's test (p < 0.05).
RESULTS: Neither classic Papacarie™ nor the modified product with a photosensitizing agent (PapaMBlue) promoted collagen degradation.
CONCLUSIONS: The irradiation of methylene blue added to papain gel with red light did not alter the chemical structure of type I collagen.

PMID: 29023186 [PubMed - as supplied by publisher]

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